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3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue
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Bioengineering
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JoVE Journal Bioengineering
3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

DOI:
10.3791/62691-v

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08:57 min

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July 16, 2021

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, , , ,
1Department of Biochemistry, Escola Paulista de Medicina,Universidade Federal de São Paulo

Chapters

  • 00:05Introduction
  • 00:49Astrocyte Isolation and Culture
  • 02:06Astrocytes-laden Gelatin/GelMA/Fibrinogen Bioink Preparation
  • 04:01Bioprinting Syringe Preparation and Bioprinting
  • 04:58Bioprinted Construct and Culture Crosslinking
  • 05:40Astrocytes Viability Assessment
  • 06:22Results: 3D Bioprinted Astrocyte Cell Viability and Morphology Assessment
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here we report a method of 3D bioprinting murine cortical astrocytes for biofabricating neural-like tissues to study the functionality of astrocytes in the central nervous system and the mechanisms involving glial cells in neurological diseases and treatments.

Tags

Keywords: 3D BioprintingAstrocytesIn Vitro ModelsNeurological DiseaseBiomaterialsExtracellular MatrixFibrinogenGelatinPhoto-initiatorLaminin

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