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JoVE Journal Neuroscience
Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery
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Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery

Article DOI: 10.3791/62698-v • 07:27 min • June 30th, 2021
June 30th, 2021 •
1, 1, 1, 1, 1, 1
1Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine, Temple University

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Summary
Automatic Translation

This protocol demonstrates a novel method for applying gene therapies to subpopulations of cells in neonatal rats at postnatal ages 5-10 days by injecting an anterograde chemogenetic modifier into the somatomotor cortex and a retrogradely transportable Cre recombinase into the cervical spinal cord.

Tags

Corticospinal Tract Neonatal Rats Double-viral Vector Combined Brain And Spine Surgery Subpopulations Of Cells Spinal Cord Injury Recovery Chemogenetic Techniques Spinal Anatomy Vertebral Segments Anesthesia Depth Incision Sagittal Suture Bregma Skull Surface
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