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Biochemistry

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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
 

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Article DOI: 10.3791/62701-v
June 29th, 2021

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Summary June 29th, 2021

We provide a detailed description of the steps required to assemble a high-pressure cell, set up and record high-pressure NMR experiments, and finally analyze both peak intensity and chemical shift changes under pressure. These experiments can provide valuable insights into the folding pathways and structural stability of proteins.

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