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A Permanent Window for Investigating Cancer Metastasis to the Lung
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Cancer Research
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JoVE Journal Cancer Research
A Permanent Window for Investigating Cancer Metastasis to the Lung

A Permanent Window for Investigating Cancer Metastasis to the Lung

DOI:
10.3791/62761-v

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07:06 min

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July 01, 2021

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, , , ,
1Department of Anatomy and Structural Biology,Einstein College of Medicine / Montefiore Medical Center, 2Gruss-Lipper Biophotonics Center,Einstein College of Medicine / Montefiore Medical Center, 3Department of Surgery,Einstein College of Medicine / Montefiore Medical Center, 4Integrated Imaging Program,Einstein College of Medicine / Montefiore Medical Cente, 5Department of Pathology,Einstein College of Medicine / Montefiore Medical Center

Chapters

  • 00:04Introduction
  • 00:59Lung Window Surgery
  • 04:46Results: Assessment of Tumor Cell Migration
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the surgical implantation of a permanently indwelling optical window for the murine thorax, which enables high-resolution, intravital imaging of the lung. The permanence of the window makes it well-suited to the study of dynamic cellular processes in the lung, especially those that are slowly evolving, such as metastatic progression of disseminated tumor cells.

Tags

Keywords: Cancer MetastasisLung MetastasisTumor Cell ColonizationTumor DormancyIn Vivo Real-time MonitoringPulmonary DiseasesIntubationRib Cage IncisionLung Window ImplantationPurse-string Suture

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