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Journal / Biochemistry / Undersøgelser af Chaperone-Cochaperone…
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Biochemistry

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JoVE Journal Biochemistry
Undersøgelser af Chaperone-Cochaperone interaktioner ved hjælp af homogen perle-baseret assay
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Journal (Biochemistry)
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Journal (Biochemistry)
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
 
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Undersøgelser af Chaperone-Cochaperone interaktioner ved hjælp af homogen perle-baseret assay

Article DOI: 10.3791/62762-v • 06:51 min • July 21st, 2021
July 21st, 2021 •
1, 1, 1,2, 1,3, 1
1Department of Neuroscience Care and Society, Division of Neurogeriatrics, Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), 3Theme Inflammation and Aging, Karolinska University Hospital

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Denne protokol præsenterer en teknik til sondering protein-protein interaktioner ved hjælp af glutathion-linked donor perler med GST-fusioneret TPR-motiv co-chaperones og acceptor perler kombineret med en Hsp90-afledt peptid. Vi har brugt denne teknik til at screene små molekyler til at forstyrre Hsp90-FKBP51 eller Hsp90-FKBP52 interaktioner og identificeret potente og selektive Hsp90-FKBP51 interaktionshæmmere.

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Biokemi Udgave 173 AlphaScreen protein-protein interaktioner molekylær chaperone Hsp90 FKBP51 FKBP52 TPR proteiner sammensatte screening
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