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Journal / Developmental Biology / A Simple and Effective Transplantation Device for …
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Developmental Biology

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JoVE Journal Developmental Biology
A Simple and Effective Transplantation Device for Zebrafish Embryos
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A Simple and Effective Transplantation Device for Zebrafish Embryos

Article DOI: 10.3791/62767-v • 06:59 min • August 2nd, 2021
August 2nd, 2021 •
1, 1, 1,2
1Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society, 2University of Konstanz

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Summary
Automatic Translation

Embryological manipulations such as extirpation and transplantation of cells are important tools to study early development. This protocol describes a simple and effective transplantation device to perform these manipulations in zebrafish embryos.

Tags

Transplantation Device Zebrafish Embryos Signaling Molecules Embryogenesis Germline Mutants Blastula Stage Medaka Embryos TDO Species Gas Tight Syringe Micro Pipette Holder Micro Manipulator Stereo Microscope Ringer's Solution Transplantation Needle Donor Embryo Orthogonal Plunger
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