Host Embryo
Host Embryo - Related Videos
02:18
Live Imaging of Host-Pathogen Interactions in Zebrafish Embryos Infected with Mycobacterium abscessus
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2026
Source: Bernut, A., et al. Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J. Vis. Exp. (2015)This video demonstrates a protocol that uses transgenic zebrafish embryos expressing fluorescent macrophages and infected with fluorescent Mycobacterium abscessus rough strain to visualize host-pathogen interactions. It reveals how extracellular cording enables immune evasion, promoting bacterial survival and host cell damage.
09:20
Fertilization of Xenopus oocytes using the Host Transfer Method
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Cited by 13 •
2010
Procedure for fertilizing Xenopus oocytes by the host transfer method.
11:14
Assay for Neural Induction in the Chick Embryo
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Cited by 1 •
2009
Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.
21:01
Generating Chimeric Zebrafish Embryos by Transplantation
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Cited by 61 •
2009
A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.
09:08
Facial Transplants in Xenopus laevis Embryos
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Cited by 10 •
2014
A technique for transplanting "Extreme Anterior Domain" facial tissue between Xenopus laevis embryos has been developed. Tissue can be moved from one gene expression background into another, allowing the study of local requirements for craniofacial development and for signaling interactions between facial regions.
FAQs
Donor cells retain intrinsic properties that affect their developmental potential, but signals from the surrounding host environment can influence how those properties are expressed. Interactions between the introduced cells and neighboring embryonic tissues therefore help determine whether cells integrate into forming structures and differentiate appropriately. This relationship allows researchers to examine how cell-internal programs and external developmental cues cooperate.
The developmental stage establishes which surrounding signals and forming tissues are available when donor cells are introduced. Because these environmental cues change during development, the same donor population may encounter different conditions at different stages. Comparing outcomes after stage-specific transplantation helps reveal how developmental timing affects cell integration, lineage behavior, tissue differentiation, and the formation of embryonic structures.
Chimera formation places donor-derived cells within a host embryo, allowing their contributions to developing tissues to be examined in relation to neighboring host cells. The tissues that contain donor cells provide evidence about where those cells can participate during development. This approach helps analyze cell potency, tissue differentiation, and the interaction between donor-cell properties and the host developmental environment.
Lineage tracing follows the contributions of introduced cells as embryonic tissues form. It can show whether donor cells remain localized, integrate into particular developing tissues, or contribute to differentiated structures. These observations connect an introduced cell population with later developmental outcomes, helping researchers investigate tissue origins, cell-fate decisions, and the processes that shape organ formation.
A typical experiment begins by selecting donor cells or tissue and a host embryo at a defined developmental stage. The donor material is then introduced into the host embryo, after which researchers examine its interactions with surrounding embryonic tissues. Subsequent analysis focuses on cell integration, tissue contribution, and differentiation, providing evidence about how the developmental environment influences fate.
These experiments can address how embryonic patterning directs cells, how organs form from developing tissues, and how cell potency changes or is expressed in a particular environment. They also help investigate developmental abnormalities by revealing where interactions between cells and their surroundings may fail. In this way, the system connects cellular behavior with larger patterns of embryonic development.