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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Article DOI: 10.3791/62799-v • 12:54 min • October 2nd, 2021
October 2nd, 2021 •
1, 1, 1, 1, 1,2, 1, 1,2
1Auckland Bioengineering Institute, University of Auckland, 2Department of Engineering Science, University of Auckland

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Summary
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This protocol presents a collection of sarcomere, calcium, and macroscopic geometric data from an actively contracting cardiac trabecula ex vivo. These simultaneous measurements are made possible by the integration of three imaging modalities.

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Brightfield Imaging Fluorescence Imaging Optical Coherence Tomography Contracting Cardiac Trabeculae Ex Vivo Imaging Mechanical Function Measurement Ionic Dynamics Measurement Geometrical Change Measurement Force Production Calcium Transients Local Displacements Shape Change Heart Disease Research Treatment Strategies Development Heterogeneity Of Muscle Activity Study Integration Of Imaging Systems Simultaneous Measurement Imaging Device Dichroic Mirrors Mounting Hooks Measurement Chamber External Trigger Line Cardiac Trabeculae Isolation And Preparation
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