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JoVE Journal Biochemistry
Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow
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Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
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Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Article DOI: 10.3791/62809-v • 08:04 min • August 16th, 2021
August 16th, 2021 •
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 7, 7,13
1AdventHelath, 2University of Illinois Chicago School of Public Health, 3Lincoln Memorial University, 4Children’s Mercy Hospital, 5Scripps Clinic, 6Cincinnati Children’s Hospital Medical Center, 7Kansas City University, 8Roblex Rex Veterans Affairs Medical Center, 9California State University San Marcos, 10East Carolina University, 11Massachusetts Institute of Technology, 12Boehringer Ingelheim Norway KS, 13Heartland Center for Mitochondrial Medicine

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Preparation of mitochondria-enriched samples from previously frozen archived solid tissues allowed the investigators to perform both functional and analytical assessments of mitochondria in various experimental modalities. This study demonstrates how to prepare mitochondria-enriched preparations from frozen heart tissue and perform analytical assessments of mitochondria.

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Mitochondrial Electron Transfer Complexes Previously Frozen Cardiac Tissue Exercise-induced Mitochondrial Bioenergetics Changes Protocol Mitochondria Samples Frozen Tissues Tissue Harvest Gentle Tissue Shredding Method Mitochondria Preparation Fully Charged Shredder Drive Unwanted Tissues Weight Measurement Heart Tissue Ice-cold Solution Blood Removal Filter Paper Towel
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