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体内通过脾T细胞流式细胞术筛选肿瘤来源的细胞外囊泡的免疫原性

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Cancer Research

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JoVE Journal Cancer Research

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

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体内通过脾T细胞流式细胞术筛选肿瘤来源的细胞外囊泡的免疫原性

DOI:

10.3791/62811-v

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08:02 min

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September 23, 2021

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Florian Stritzke², Hendrik Poeck^2,3,4, Simon Heidegger²

¹Department of Medicine III, School of Medicine,Technical University of Munich, ²Center for Translational Cancer Research (TranslaTUM), School of Medicine,Technical University of Munich, ³Department of Internal Medicine III,University Hospital Regensburg, ⁴National Centre for Tumor Diseases WERA

Chapters

00:05Introduction
00:48Generation and Isolation of Extracellular Vesicles (EVs) Derived from Tumor Cells After Chemotherapy Exposure
02:59Immunization of C57BL/6 Mice with Extracellular Vesicles (EVs) and Flow Cytometry Analysis of Splenic T Cells
06:01Results: The Assessment of the Immunogenicity of Tumor-Derived Extracellular Vesicles (EVs)
07:21Conclusion

Summary

Automatic Translation

本手稿描述了如何使用流式细胞术评估肿瘤细胞来源的细胞外囊泡（EV）的体内免疫原性。来自接受治疗诱导的免疫原性细胞死亡的肿瘤的EV似乎与肿瘤免疫监测特别相关。该协议是奥沙利铂诱导的免疫刺激性肿瘤EV评估的例证，但可以适应各种环境。

Tags

– Tumor-derived Extracellular Vesicles- Flow Cytometry- Splenic T Cells- In Vivo Immunogenicity Screening- B16 Melanoma Cells- Ovalbumin- Oxaliplatin- Ultracentrifugation- EV Isolation- Exosome Isolation Reagent- Immunization

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