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Immunology and Infection
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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

DOI:
10.3791/62857-v

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07:59 min

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September 10, 2021

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,
1Department of Infectious Diseases, Molecular Virology,Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine,University of Florida, Gainesville, 3Department of Infectious Diseases, Virology,Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”,German Cancer Research Center (DKFZ)

Chapters

  • 00:04Introduction
  • 01:04Preparation of Single-Cell Solution and Preparation of Gel Beads-in-Emulsion Under Biosafety Level 3 Conditions
  • 05:47Results: Characterization of Host/Pathogen Interactions at a Single-Cell Level Using scRNAseq Technology
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to infect human intestinal organoids from either their apical or basolateral side to characterize host/pathogen interactions at the single-cell level using single-cell RNA sequencing (scRNAseq) technology.

Tags

Keywords: Gastrointestinal OrganoidsPathogen InfectionSingle Cell SequencingBiosafety Level 3Viral InfectionIntestinal EpitheliumDifferentiated OrganoidsExtracellular MatrixDissociation EnzymeSingle Cell RNA Sequencing

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