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JoVE Journal
Genetics

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使用短读和长读测序技术生成尿细菌完整基因组的杂交从 基因组组装
 
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使用短读和长读测序技术生成尿细菌完整基因组的杂交从 基因组组装

Article DOI: 10.3791/62872-v 12:08 min August 20th, 2021
August 20th, 2021

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Summary

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Click here for the English version.

该协议详细介绍了尿细菌培养,测序和 从头 杂交基因组组装的综合方法。它为生成完整的圆形基因组序列提供了一种可重复的程序,可用于研究有助于尿定植、发病机制和抗菌素耐药性传播的染色体和染色体外遗传元件。

Tags

遗传学,第174期,尿路感染,细菌,杂交基因组组装,纳米孔测序,16S rRNA,下一代测序,移动遗传元件,抗菌素耐药性
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