Journal
/
Neuroscience
/
Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal

Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal

DOI:
10.3791/62883-v

•

12:04 min

•

September 10, 2021

•

, , , ,
1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London

Chapters

  • 00:04Introduction
  • 00:38SPECT Camera Preparation and Calibration
  • 01:47[125I]CLINDE Radiotracer Synthesis
  • 04:27SPECT Acquisition
  • 05:15Scan Reconstruction
  • 05:57Rat Brain Extraction and Cell Isolation
  • 09:46Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) is a powerful tool to study the role of the 18 kDa translocator protein or Serotonin 5HT2A-receptor expression in Alzheimer's Disease at a cellular scale. This protocol describes the ex-vivo application of FACS-RTT in the TgF344-AD rat model.

Tags

Keywords: FACS-RTTFluorescence-activated Cell SortingRadioligandIn Vivo ImagingRadiotracerCell TypeCellular OriginRadioactive SignalCell SortingSensitivityCellular ResolutionPhantom ScanRadioactivityCLINDEReverse-phase ColumnHPLCConcentration ColumnStock SolutionCalibration CurvesLinear Regression

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62883?article_type=v

An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice
check_url/62883?article_type=v

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
check_url/62883?article_type=v

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
check_url/62883?article_type=v

An In Vitro Model for the Study of Cellular Pathophysiology in Globoid Cell Leukodystrophy
check_url/62883?article_type=v

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
check_url/62883?article_type=v

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
check_url/62883?article_type=v

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
check_url/62883?article_type=v

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
check_url/62883?article_type=v

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae
check_url/62883?article_type=v

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Read Article