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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal

Article DOI: 10.3791/62883-v • 12:04 min • September 10th, 2021
September 10th, 2021 •
1,2, 1,3,4, 1,5, 1,2, 1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London

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Summary
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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) is a powerful tool to study the role of the 18 kDa translocator protein or Serotonin 5HT2A-receptor expression in Alzheimer's Disease at a cellular scale. This protocol describes the ex-vivo application of FACS-RTT in the TgF344-AD rat model.

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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue FACS-RTT In Vivo Imaging Radiotracer Quantification Cellular Resolution Radioligands Cell Sorting Camera Operating Software XYZ Stage Button Homing Scan Acquisition Step Mode Listmode Emission Spectrum Bed Setup Warming System Breathing Sensor Anesthesia Phantom Positioning Scan Area Calibration Appropriate Environment Radioactivity Experiments Tributylin Precursor Acetic Acid Sodium Iodide Paracetic Acid Thermocycler
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