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JoVE Journal Neuroscience
Fluorescensaktiverad cellsortering-radioligandbehandlad vävnad (FACS-RTT) för att bestämma det cellulära ursprunget för radioaktiv signal
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Fluorescensaktiverad cellsortering-radioligandbehandlad vävnad (FACS-RTT) för att bestämma det cellulära ursprunget för radioaktiv signal

Article DOI: 10.3791/62883-v • 12:04 min • September 10th, 2021
September 10th, 2021 •
1,2, 1,3,4, 1,5, 1,2, 1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London

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Fluorescensaktiverad cellsortering-radioligandbehandlad vävnad (FACS-RTT) är ett kraftfullt verktyg för att studera rollen av 18 kDa-translokatorproteinet eller Serotonin 5HT2A-receptoruttrycket vid Alzheimers sjukdom i cellulär skala. Detta protokoll beskriver ex-vivo-appliceringen av FACS-RTT i TgF344-AD-råttmodellen.

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Neurovetenskap Utgåva 175 Alzheimers sjukdom Fluorescensaktiverad cellsortering till radioligand treaded vävnader TgF344-AD Positronemissionstomografi / Single Photon Emission Computed Tomography TSPO 5HT2AR
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