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Messung des mitochondrialen Substratflusses in rekombinanten Perfringolysin O-permeabilisierten Zellen
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Messung des mitochondrialen Substratflusses in rekombinanten Perfringolysin O-permeabilisierten Zellen

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

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In dieser Arbeit beschreiben wir ein modifiziertes Protokoll zum Testen des mitochondrialen respiratorischen Substratflusses unter Verwendung von rekombinantem Perfringolysin O in Kombination mit mikroplattenbasierter Respirometrie. Mit diesem Protokoll zeigen wir, wie Metformin die mitochondriale Atmung von zwei verschiedenen Tumorzelllinien beeinflusst.

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Biologie Ausgabe 174 Mikroplatten-Respirometrie Mitochondrien Metformin permeabilisierte Zellen Perfringolysin O mitochondriale Substrate
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