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Journal / Biology / Rekombinant Perfringolysin O-Permeabilized…
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Rekombinant Perfringolysin O-Permeabilized Hücrelerde Mitokondriyal Substrat Akılarının Ölçülmesi
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Rekombinant Perfringolysin O-Permeabilized Hücrelerde Mitokondriyal Substrat Akılarının Ölçülmesi

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

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Bu çalışmada, mikro plaka bazlı respirometri ile birlikte rekombinant perfringolysin O kullanarak mitokondriyal solunum substrat akısını test etmek için değiştirilmiş bir protokol açıklıyoruz. Bu protokol ile metforminin iki farklı tümör hücre hattının mitokondriyal solunumunu nasıl etkilediğini gösteriyoruz.

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Biyoloji Sayı 174 mikro plaka respirometrisi mitokondri metformin permeabilize hücreler perfringolysin O mitokondriyal substratlar
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