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Journal / Biochemistry / 时间分辨Förster共振能量转移测定法，用于测量人细胞中的内源性磷酸化STAT蛋白…
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JoVE Journal Biochemistry
时间分辨Förster共振能量转移测定法，用于测量人细胞中的内源性磷酸化STAT蛋白
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gP2S, an Information Management System for CryoEM Experiments
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Journal (Biochemistry)
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时间分辨Förster共振能量转移测定法，用于测量人细胞中的内源性磷酸化STAT蛋白

Article DOI: 10.3791/62915-v • 07:12 min • September 9th, 2021
September 9th, 2021 •
1, 1, 1
1BioAuxilium Research

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描述了基于时间分辨Förster共振能量转移细胞的测定方案，用于以384孔格式对细胞裂解物中内源性磷酸化信号转导和转录激活子（STAT）1/3/4/5/6蛋白的有效定量进行简单，特异性，灵敏和稳健的定量。

Tags

生物化学，第175期，JAK / STAT信号传导，STAT蛋白，磷酸化，均质测定，TR-FRET，免疫测定，基于细胞的测定，激酶，细胞信号传导，高通量筛选，细胞因子信号传导
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