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Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)
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JoVE Journal Neuroscience
Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)

Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)

DOI:
10.3791/62917-v

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07:46 min

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January 22, 2022

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, , ,
1Dalton Cardiovascular Research Center,University of Missouri-Columbia, 2Department of Biomedical, Biological and Chemical Engineering,University of Missouri-Columbia

Chapters

  • 00:03Introduction
  • 00:38Construction of DNA Plasmids
  • 00:57In vitro Mitochondrial Ca2+ Imaging in Astrocytes and Neurons
  • 01:32In vivo Mitochondrial Ca2+ Imaging in Astrocytes and Neurons
  • 03:51Cranial Window Installation and in vivo 2-P Imaging of Mitochondrial Ca2+ Signals
  • 05:42Representative Results of in vitro and in vivo Mitochondrial Ca2+ Imaging
  • 07:24Conclusions

Summary

Automatic Translation

Automatic Translation

This proctocol aims to provide a method for in vitro and in vivo mitochondrial Ca2+ imaging in astrocytes and neurons.

Tags

Keywords: Mitochondrial Calcium ImagingAstrocytesNeuronsGenetically Encoded Calcium Indicators (GECIs)GCaMP5GGCaMP6sIn VitroIn VivoAdenovirusStereotaxic InjectionMotor CortexParacortex

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