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使用遗传编码的Ca
2 +
指示剂(GECI)对星形胶质细胞和神经元中的线粒体Ca
2 +
摄取进行成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Imaging Mitochondrial Ca
2+
Uptake in Astrocytes and Neurons using Genetically Encoded Ca
2+
Indicators (GECIs)
Please note that all translations are automatically generated.
Click here for the English version.
使用遗传编码的Ca
2 +
指示剂(GECI)对星形胶质细胞和神经元中的线粒体Ca
2 +
摄取进行成像
DOI:
10.3791/62917-v
•
07:46 min
•
January 22, 2022
•
Nannan Zhang
,
Zhe Zhang
2
,
Ilker Ozden
,
Shinghua Ding
2
1
Dalton Cardiovascular Research Center
,
University of Missouri-Columbia
,
2
Department of Biomedical, Biological and Chemical Engineering
,
University of Missouri-Columbia
Chapters
00:03
Introduction
00:38
Construction of DNA Plasmids
00:57
In vitro
Mitochondrial Ca
2+
Imaging in Astrocytes and Neurons
01:32
In vivo
Mitochondrial Ca
2+
Imaging in Astrocytes and Neurons
03:51
Cranial Window Installation and
in vivo
2-P Imaging of Mitochondrial Ca
2+
Signals
05:42
Representative Results of
in vitro
and
in vivo
Mitochondrial Ca
2+
Imaging
07:24
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
该proctocol旨在为星形胶质细胞和神经元
中的体外
和
体内
线粒体Ca
2 +
成像提供一种方法。
Tags
Mitochondrial Calcium Imaging
Astrocytes
Neurons
Genetically Encoded Calcium Indicators (GECIs)
GCaMP5G
GCaMP6s
In Vitro
In Vivo
Adenovirus
Stereotaxic Injection
Motor Cortex
Paracortex
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