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Journal / Neuroscience / Avbildning av mitokondrie Ca2+ opptak i…
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Neuroscience
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JoVE Journal Neuroscience
Avbildning av mitokondrie Ca2+ opptak i astrocytter og nevroner ved hjelp av genetisk kodede Ca2+ indikatorer (GECIer)
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Avbildning av mitokondrie Ca2+ opptak i astrocytter og nevroner ved hjelp av genetisk kodede Ca2+ indikatorer (GECIer)

Article DOI: 10.3791/62917-v • 07:46 min • January 22nd, 2022
January 22nd, 2022 •
1, 1,2, 2, 1,2
1Dalton Cardiovascular Research Center, University of Missouri-Columbia, 2Department of Biomedical, Biological and Chemical Engineering, University of Missouri-Columbia

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Denne proctocol har som mål å gi en metode for in vitro og in vivo mitokondrie Ca2 + avbildning i astrocytter og nevroner.

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Nevrovitenskap utgave 179 astrocytt nevron mitokondrier GECIer GCaMP5G/6s mitokondrier-målrettingssekvens gfaABC1D-promotor CaMKII-promotor in vivo 2-fotonavbildning
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