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使用多功能系统对人类癌症和正常细胞中Yes相关蛋白的全光学机械生物学询问
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
Please note that all translations are automatically generated.
Click here for the English version.
使用多功能系统对人类癌症和正常细胞中Yes相关蛋白的全光学机械生物学询问
DOI:
10.3791/62934-v
•
09:55 min
•
December 20, 2021
•
Qin Luo*
1
,
Miao Huang*
2
,
Chenyu Liang
,
Justin Zhang
,
Gaoming Lin
,
Sydney Yu
,
Mai Tanaka
5
,
Sharon Lepler
5
,
Juan Guan
6,7
,
Dietmar Siemann
5
,
Xin Tang
5
1
Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering
,
University of Florida
,
2
Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering
,
University of Florida
,
3
Department of Electrical and Computer Engineering
,
University of California
,
4
Department of Radiation Oncology, College of Medicine
,
University of Florida
,
5
UF Health Cancer Center
,
University of Florida
,
6
Department of Physics, College of Liberal Arts and Sciences
,
University of Florida
,
7
Department of Anatomy and Cell Biology, College of Medicine
,
University of Florida
Chapters
00:04
Introduction
00:53
Cell Imaging
07:21
Results: All-Optical Mechanobiology Interrogation of Yes-Associated Protein in Human Cancer and Normal Cells
09:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了如何利用集成的多功能和用户可编程系统的详细逐步方案,该系统可实现自动多通道成像和机械生物学分析,以阐明Yes相关蛋白(YAP)的机械灵敏度。
Tags
Mechanobiology
Yes-associated Protein
Cancer Cells
Normal Cells
Multi-functional System
Optical Electrophysiology
Super-resolution Imaging
Live-cell Imaging
High-throughput
Self-drift Correcting
Long-term Imaging
Fluorescent Microscopy
NYCOM
Environment Chamber
Motorized Stage
CO2 Flow
Temperature Control
Glass-bottom Petri Dish
Confocal Controller
MicroManager
Adhesive Tape
Adhesive Glue
IntelliJ
AMFIP
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