A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

Kyoko Yarimizu; So Fujiyoshi; Mikihiko Kawai; Jacquelinne J. Acu&#241;a; Joaquin-Ignacio Rilling; Marco Campos; Jonnathan Vilugr&#243;n; Henry Cameron; Karen Vergara; Gonzalo Gajardo; Oscar Espinoza-Gonz&#225;lez; Leonardo Guzm&#225;n; Satoshi Nagai; Carlos Riquelme; Milko A. Jorquera; Fumito Maruyama

doi:10.3791/62967

Journal

/

Environment

/

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

JoVE Journal
Environment

This content is Free Access.

JoVE Journal Environment

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

5,145 Views

•

09:47 min

•

August 26, 2021

DOI:

10.3791/62967-v

DOI:

10.3791/62967-v

•

09:47 min

August 26, 2021

•

5138 Views

Kyoko Yarimizu¹, So Fujiyoshi¹, Mikihiko Kawai², Jacquelinne J. Acuña³, Joaquin-Ignacio Rilling³, Marco Campos³, Jonnathan Vilugrón⁴, Henry Cameron⁵, Karen Vergara⁶, Gonzalo Gajardo⁶, Oscar Espinoza-González⁴, Leonardo Guzmán⁴, Satoshi Nagai⁷, Carlos Riquelme⁵, Milko A. Jorquera³, Fumito Maruyama¹

¹Office of Research and Academia-Government-Community Collaboration,Hiroshima University ²Graduate School of Human and Environmental Studies,Kyoto University ³Scientific and Biotechnological Bioresource Nucleus,Universidad de La Frontera ⁴Centro de Estudios de Algas Nocivas,Instituto de Fomento Pesquero ⁵Ciencias del Mar y Recursos Biologicos,Universidad de Antofagasta ⁶Departamento de Ciencias Biológicas y Biodiversidad,Universidad de Los Lagos ⁷Japan Fisheries Research and Education Agency,Fisheries Resources Institute

Summary

This protocol introduces steps of metabarcoding analysis, targeting 16S rRNA and 18S rRNA genes, for monitoring harmful algal blooms and their associated microbiome in seawater samples. It is a powerful molecular-based tool but requires several procedures, which are visually explained here step-by-step.