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JoVE Journal Neuroscience
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
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Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Article DOI: 10.3791/63007-v • 07:14 min • October 21st, 2021
October 21st, 2021 •
1,2,3, 1,2,3, 1,2,3, 1,2, 1,2,3, 1,2,3
1Division of Radiation Health, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences

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Summary
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This neural cell dissociation protocol is intended for samples with a low amount of starting material and yields a highly viable single-cell suspension for downstream analysis, with optional fixation and staining steps.

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