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JoVE Journal Bioengineering
Une boîte à outils de transcription-traduction de streptomyces à haut rendement pour la biologie synthétique et les applications de produits naturels
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Une boîte à outils de transcription-traduction de streptomyces à haut rendement pour la biologie synthétique et les applications de produits naturels

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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Ce protocole détaille une méthode améliorée pour synthétiser des rendements élevés de protéines recombinantes à partir d’un système de transcription-traduction sans cellule (TX-TL) de Streptomyces venezuelae .

Tags

Bioingénierie Numéro 175 Synthèse de protéines sans cellules transcription-traduction in vitro Streptomyces biologie synthétique biologie des systèmes systèmes sans cellules biosynthèse
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