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Biology
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通过粘附足迹测定法对细胞滚动中的分子粘附进行成像
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Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
Please note that all translations are automatically generated.
Click here for the English version.
通过粘附足迹测定法对细胞滚动中的分子粘附进行成像
DOI:
10.3791/63013-v
•
08:24 min
•
September 27, 2021
•
Scott Minh An*
1,2
,
Seong Ho Kim*
1
,
Vanessa J. White*
1,2
,
Adam B. Yasunaga*
1,2
,
Kathleen M. McMahon
,
Yousif Murad
3
,
Isaac T. S. Li
2
1
Department of Chemistry
,
The University of British Columbia
,
2
Biochemistry and Molecular Biology
,
The University of British Columbia
,
3
Faculty of Medicine
,
The University of British Columbia
Chapters
00:04
Introduction
00:57
Flow Chamber Preparation
01:52
Surface Preparation
03:14
Experiment and Imaging
05:52
Results: The Analysis of Image Processing, Suboptimal and Optimal Results, and Bioconjugation Characterization
07:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了执行粘附足迹测定的实验程序,以在快速细胞滚动粘合期间对粘附事件进行成像。
Tags
Imaging Molecular Adhesion
Cell Rolling
Adhesion Footprint Assay
Molecular Force
Surface Functionalization
Bioconjugation
Flow Chamber
Epoxy-coated Tape
Wash Buffer
BSA
Tween 20
Streptavidin
Protein G-TGT
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