JoVE Journal
Biochemistry
Biochemistry
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Summary
Please note that all translations are automatically generated.
该协议描述了一种微型基因报告基因检测,用于监测5'-剪接位点突变对剪接的影响,并开发抑制因子U1 snRNA以挽救突变诱导的剪接抑制。报告基因和抑制子U1 snRNA构建体在HeLa细胞中表达,并通过引物延伸或RT-PCR分析剪接。