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JoVE Journal
Biochemistry

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一种基于报告基因的细胞检测,用于监测剪接效率
 
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一种基于报告基因的细胞检测,用于监测剪接效率

Article DOI: 10.3791/63014-v 08:53 min September 15th, 2021
September 15th, 2021

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Summary

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Click here for the English version.

该协议描述了一种微型基因报告基因检测,用于监测5'-剪接位点突变对剪接的影响,并开发抑制因子U1 snRNA以挽救突变诱导的剪接抑制。报告基因和抑制子U1 snRNA构建体在HeLa细胞中表达,并通过引物延伸或RT-PCR分析剪接。

Tags

生物化学, 第175期, RNA-蛋白相互作用, 前mRNA剪接, 剪接体, U1 snRNP, 内含子, 外显子, 剪接位点, Dup51p报告基因, 外显子跳跃
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