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Et reporterbaseret cellulært assay til overvågning af splejsningseffektivitet
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Et reporterbaseret cellulært assay til overvågning af splejsningseffektivitet

Article DOI: 10.3791/63014-v • 08:53 min • September 15th, 2021
September 15th, 2021 •
1, 1, 1
1Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

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Denne protokol beskriver et minigen reporter-assay for at overvåge virkningen af 5'-splejsningsstedsmutationer på splejsning og udvikler suppressor U1 snRNA til redning af mutationsinduceret splejsningshæmning. Reporter- og suppressoren U1 snRNA-konstruktioner udtrykkes i HeLa-celler, og splejsning analyseres ved primerudvidelse eller RT-PCR.

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Biokemi Udgave 175 RNA-proteininteraktion præ-mRNA-splejsning spliceosom U1 snRNP intron exon splejsningssted Dup51p-reporter exon-springning
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