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Biology
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检测植物宿主中叶蝉载体的病毒和唾液蛋白
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Biology
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JoVE Journal
Biology
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
Please note that all translations are automatically generated.
Click here for the English version.
检测植物宿主中叶蝉载体的病毒和唾液蛋白
DOI:
10.3791/63020-v
•
07:23 min
•
September 14, 2021
•
Yanfei Wang
,
Xin Wang
,
Zhiqiang Li
,
Qian Chen
1
Vector-borne Virus Research Center, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology
,
Fujian Agriculture and Forestry University
Chapters
00:05
Introduction
00:35
Non-Viruliferous Insect Rearing
01:29
Virus Acquisition and Collection of Viruliferous Insects
02:10
Collection of Salivary Proteins Using a Feeding Cage
03:04
Reagent Preparation
03:51
Detection of Saliva and Viral Proteins by Western Blotting
06:02
Results: Western Blot Analysis for Detection of Viral and Salivary Proteins of a Leafhopper Vector in the Plant Host
06:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议演示了如何使用植物宿主来检测叶蝉的唾液蛋白和叶蝉载体释放的植物病毒蛋白。
Tags
Leafhopper
Salivary Proteins
Plant Host
Virus Detection
Virus Transmission
Rice Dwarf Virus
Feeding Cage
Aspirator
Non-viruliferous Nymphs
Circulative Transmission
Insect-proof Net
Tris-Glycine Buffer
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