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Rapid Isolation of Single Cells from Mouse and Human Teeth
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

DOI:
10.3791/63043-v

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06:09 min

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October 28, 2021

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, , ,
1Department of Histology and Embryology, Faculty of Medicine,Masaryk University, 2Department of Molecular Neuroimmunology, Centre for Brain Research,Medical University of Vienna, 3Department of Physiology and Pharmacology,Karolinska Institute

Chapters

  • 00:05Introduction
  • 01:09Tissue Dissection
  • 02:58Single-Cell Suspension Preparation
  • 04:42Results: Quantification of the Immune Cells
  • 05:24Conclusion

Summary

Automatic Translation

Automatic Translation

The current protocol presents a fast, efficient, and gentle method for isolating single cells suitable for single-cell RNA-seq analysis from a continuously growing mouse incisor, mouse molar, and human teeth.

Tags

Keywords: Single-cell IsolationMouse TeethHuman TeethCell SuspensionDental PulpMandibleEuthanasiaHBSSTweezersScalpelCell Quality

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