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Journal / Biology / 使用细胞外通量分析仪评估小鼠造血干细胞和原始祖细胞中的细胞生物能量学…
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使用细胞外通量分析仪评估小鼠造血干细胞和原始祖细胞中的细胞生物能量学
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使用细胞外通量分析仪评估小鼠造血干细胞和原始祖细胞中的细胞生物能量学

Article DOI: 10.3791/63045-v • 10:17 min • September 24th, 2021
September 24th, 2021 •
1, 2, 2,3,4, 1,4,5
1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor

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这里介绍的方法总结了使用细胞外通量分析仪实时测量HSPCs的细胞外酸化速率（ECAR）和耗氧速率（OCR）评估非贴壁小鼠造血干细胞和原始祖细胞（HSPC）中细胞生物能量的优化方案。

Tags

生物学，第175期，造血干细胞，造血祖细胞，糖酵解，线粒体呼吸，氧化磷酸化，细胞外酸化速率，耗氧率，细胞外通量，非贴壁细胞
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