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Journal
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Biochemistry
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免疫肽组学:分离小鼠和人MHC I类和II类相关肽用于质谱分析
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Please note that all translations are automatically generated.
Click here for the English version.
免疫肽组学:分离小鼠和人MHC I类和II类相关肽用于质谱分析
DOI:
10.3791/63052-v
•
09:32 min
•
October 15, 2021
•
Isabelle Sirois*
1
,
Maxim Isabelle*
1
,
Jérôme D. Duquette
,
Frederic Saab
,
Etienne Caron
2
1
CHU Sainte-Justine Research Center
,
2
Department of Pathology and Cellular Biology, Faculty of Medicine
,
Université de Montréal
Chapters
00:04
Introduction
00:41
Coupling Antibodies to Sepharose CNBr-Activated Beads
02:57
Cell Lysis and Immunocapture with Antibody Coupled-Beads
04:06
Elution of MHC Peptides
07:11
Results: Analysis of the MHC-Binding Affinity
08:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一种从小鼠和人细胞系纯化MHC I类和II类肽复合物的方案,提供高质量的免疫肽组学数据。该协议侧重于使用市售抗体进行样品制备。
Tags
Immunopeptidomics
MHC Class I
MHC Class II
Peptide Isolation
Mass Spectrometry
Human
Mouse
Autoimmune Diseases
Cancer
Virology
Sepharose Beads
Antibody Coupling
Blocking
Washing
Cell Lysis
Peptide Elution
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