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Journal / Biochemistry / Immunopeptidomics: Isolering av mus och mänskliga …
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Biochemistry

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JoVE Journal Biochemistry
Immunopeptidomics
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Immunopeptidomics: Isolering av mus och mänskliga MHC klass I- och II-associerade peptider för massspektrometrianalys

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Här presenterar vi ett protokoll för rening av MHC klass I och klass II peptidkomplex från mus och mänskliga cellinjer som ger högkvalitativa immunpeptidomics data. Protokollet fokuserar på provberedning med kommersiellt tillgängliga antikroppar.

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Biokemi nummer 176
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