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Journal / Biochemistry / İmmünopeptidomik: Kütle Spektrometresi Analizi…
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Biochemistry

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JoVE Journal Biochemistry
İmmünopeptidomik
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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Journal (Biochemistry)
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İmmünopeptidomik: Kütle Spektrometresi Analizi için Fare ve İnsan MHC Sınıfı I ve II İlişkili Peptitlerin İzolasyonu

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Burada, MHC sınıf I ve sınıf II peptit komplekslerinin yüksek kaliteli immünopeptidomik veriler sağlayan fare ve insan hücre çizgilerinden arındırılması için bir protokol sunuyoruz. Protokol, piyasada bulunan antikorları kullanarak numune hazırlamaya odaklanır.

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