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Journal
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Neuroscience
/
原位
急性
离体
胚胎脑切片培养物中轴突生长和生长锥动力学的可视化
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
In Situ
Visualization of Axon Growth and Growth Cone Dynamics in Acute
Ex Vivo
Embryonic Brain Slice Cultures
Please note that all translations are automatically generated.
Click here for the English version.
原位
急性
离体
胚胎脑切片培养物中轴突生长和生长锥动力学的可视化
DOI:
10.3791/63068-v
•
10:45 min
•
October 14, 2021
•
Eissa Alfadil
,
Frank Bradke
,
Sebastian Dupraz
1
Laboratory of Axon Growth and Regeneration
,
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Chapters
00:05
Introduction
01:11
Embryo Extraction
01:44
Ex Utero and In Utero Electroporation
03:35
Brain Extraction and Embedding in Agarose
04:39
Organotypic Slice Culture
05:43
Image Acquisition and Data Analysis
08:29
Results: In Situ Axon Growth and Growth Cone Dynamics
09:56
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
该协议展示了一种直接而稳健
的方法来研究原位
轴突生长和生长锥动态。它描述了如何制备
离体
生理相关的急性脑切片,并提供了用户友好的分析管道。
Tags
Axon Growth
Growth Cone Dynamics
Ex Vivo
Embryonic Brain Slice Cultures
In Situ Visualization
DNA Delivery
Mouse Embryonic Brain
Organotypic Culture
Axon Plasticity
Traumatic Conditions
Pathological Conditions
Electroporation
Brain Dissection
Slicing
Ex Utero Electroporation
In Utero Electroporation
Lateral Ventricle
Platinum Tweezer Electrodes
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