JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
使用PCR板深孔微孔设备生成更大的细菌生物膜生物质
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
Please note that all translations are automatically generated.
Click here for the English version.
使用PCR板深孔微孔设备生成更大的细菌生物膜生物质
DOI:
10.3791/63069-v
•
10:57 min
•
April 22, 2022
•
Ali N. Doucet
,
Carmine J. Slipski
,
George R. Golding
2
,
Michael R. Mulvey
2
,
Denice C. Bay
1
Department of Medical Microbiology and Infectious Diseases
,
University of Manitoba
,
2
National Microbiology Laboratory
,
Public Health Agency of Canada
Chapters
00:15
Introduction
01:41
Days 0-2: Aseptic Culture Preparation for Biofilm Group
02:05
Day 2: Preparation and Inoculation of Deep Well Biofilm Plates
03:08
Day 3, Part 1: Biofilm Biomass Determination from Device Using CV Staining
04:10
Day 3, Part 2: Challenging Biofilms with Antimicrobial to Calculate Their 24 Hour Antimicrobial MBEC Value
04:54
Day 4: Recovery of Biofilm Biomass from Pegged Lids and Day 5: Determination of Device MBEC Values
05:44
Representative Results
09:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议提供了使用自组装的深孔PCR板设备进行生物膜生长和生物质测量的方法,用于高通量96孔钉住盖子静态生物膜筛选。
Tags
Bacterial Biofilm
Biofilm Biomass
PCR-plate Deep Well Microplate
Peg Lid Biofilm Device
Crystal Violet Staining
Antimicrobial Susceptibility Testing
Minimum Biofilm Eradication Concentration (MBEC)
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
酶标菜生物膜形成实验
在体外
生物膜的形成以及8商会幻灯片
钩端螺旋体暴露于表面的蛋白免疫荧光法
测量人体血浆中的凝血因子V活性,用酶联凝固分析
唾液,唾液腺,淋巴收集肩突硬蜱蜱
生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体
针对相关生物膜<em>金黄色葡萄球菌</em>使用基于刃天青药物敏感性测定
可视化痰生物膜发展影响使用腔室盖玻片型号
全自动微流控装置在
白色念珠菌
生物膜形成中的可视化
基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响
Read Article