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使用PCR板深孔微孔设备生成更大的细菌生物膜生物质
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal Immunology and Infection
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
DOI:

10:57 min

April 22, 2022

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Chapters

  • 00:15Introduction
  • 01:41Days 0-2: Aseptic Culture Preparation for Biofilm Group
  • 02:05Day 2: Preparation and Inoculation of Deep Well Biofilm Plates
  • 03:08Day 3, Part 1: Biofilm Biomass Determination from Device Using CV Staining
  • 04:10Day 3, Part 2: Challenging Biofilms with Antimicrobial to Calculate Their 24 Hour Antimicrobial MBEC Value
  • 04:54Day 4: Recovery of Biofilm Biomass from Pegged Lids and Day 5: Determination of Device MBEC Values
  • 05:44Representative Results
  • 09:07Conclusion

Summary

Automatic Translation

该协议提供了使用自组装的深孔PCR板设备进行生物膜生长和生物质测量的方法,用于高通量96孔钉住盖子静态生物膜筛选。

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