A subscription to JoVE is required to view this content. Sign in or start your free trial.
1School of Women’s and Children’s Health, Faculty of Medicine and Health,University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health,University of New South Wales, 3Department of Respiratory Medicine,Sydney Children’s Hospital, 4Department of Pathology, School of Medical Sciences,University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre,University of New South Wales
Chapters
- 00:05Introduction
- 01:16Collection of Nasal Inferior Turbinate Brushings
- 02:25Airway Epithelial Cell Expansion and Maintenance
- 03:23Seeding and Differentiation of Airway Epithelial Cells and Maintenance of Differentiated ALI Models
- 04:12Three-Dimensional Airway Epithelial Organoids
- 05:54Imaging Cilia Beat Frequency
- 07:18Data Analysis and Quantification of Cilia Beat Frequency
- 09:20Results: Cilia Beat Frequency in Airway Epithelial Cell ALI Models of Cystic Fibrosis Patients and Healthy Participants
- 10:36Conclusion
Questo protocollo descrive la raccolta, l'espansione e la differenziazione delle cellule epiteliali nasali in modelli organotipici di cellule epiteliali delle vie aeree e la quantificazione della frequenza del battito delle ciglia tramite imaging di cellule vive e script personalizzati.
