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JoVE Journal
Biochemistry

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Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
 

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Article DOI: 10.3791/63092-v
October 28th, 2021

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Summary October 28th, 2021

Detailed protocol and three Python scripts are provided for operating an open-source robotic liquid handling system to perform semi-automated protein sample preparation for mass spectrometry experiments, covering detergent removal, protein digestion, and peptide desalting steps.

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