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Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives
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Neuroscience
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JoVE Journal Neuroscience
Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

DOI:
10.3791/63116-v

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06:09 min

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November 08, 2021

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, , ,
1Department of Clinical Medicine (K1),University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases,Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine,Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling

Chapters

  • 00:04Introduction
  • 00:54Flow Cytometry Measurement of Mitochondrial Volume, MMP, and Mitochondrial ROS in Live Cells
  • 02:26Flow Cytometry Measurement of MRC Complex Subunits and TFAM in Fixed Cells
  • 03:49Flow Cytometry Analysis
  • 04:14Results: Flow Cytometry Investigation of iPSC-Derived Neural Cells
  • 05:07Conclusion

Summary

Automatic Translation

Automatic Translation

This study reports a novel approach to measure multiple mitochondrial functional parameters based on flow cytometry and double staining with two fluorescent reporters or antibodies to detect changes in mitochondrial volume, mitochondrial membrane potential, reactive oxygen species level, mitochondrial respiratory chain composition, and mitochondrial DNA.

Tags

Keywords: Flow CytometryMitochondrial ParametersHuman Induced Pluripotent Stem CellsNeural DerivativesGlial DerivativesFCCPTMREMTGMito SoxCell DissociationParaformaldehydeMethanolBlocking Buffer

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