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JoVE Journal
Biology

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原发性患者特异性主动脉平滑肌细胞的分离和体外半定量实时收缩测量
 
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原发性患者特异性主动脉平滑肌细胞的分离和体外半定量实时收缩测量

Article DOI: 10.3791/63122-v 08:28 min February 15th, 2022
February 15th, 2022

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Summary

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Click here for the English version.

本文介绍了一种基于外植体培养的方法,用于分离和培养原代患者特异性人主动脉平滑肌细胞和真皮成纤维细胞。此外,还提出了一种用于测量细胞收缩和后续分析的新方法,可用于研究这些细胞中患者特异性差异。

Tags

生物学,第 180 期,平滑肌细胞、主动脉瘤、收缩、转化、体外、收缩试验、患者特异性原发性细胞分离
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