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JoVE Journal Cancer Research
Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection
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Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

Article DOI: 10.3791/63123-v • 05:49 min • November 1st, 2021
November 1st, 2021 •
1, 1, 1, 1
1Department of Experimental and Clinical Medicine, Section of Internal Medicine, University of Florence

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Summary
Automatic Translation

We present a protocol to generate a minimally invasive orthotopic pancreatic cancer model by ultrasound-guided injection of human pancreatic cancer cells and the subsequent monitoring of tumor growth in vivo by ultrasound imaging.

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Orthotopic Xenograft Pancreatic Cancer Cells Ultrasound-guided Injection Mouse Model Engraftment Tumor Development Reproducibility Minimally Invasive Surgical Procedure Drawbacks 3Rs Principles Echo-guided Injection Biliary Tree Injections Breast Cancer Models Small Molecules 8-week-old Female Mice Tramadol Administration Ultrasound Imaging B-Mode Imaging
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