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A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Article DOI: 10.3791/63134-v • 09:21 min • November 30th, 2021
November 30th, 2021 •
*1, *1, 1, 2, 3, 3, 3, 2, 1, 1, 1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.

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Triple Culture Cell System Human Blood-Brain Barrier Drug Delivery Nanomaterials In Vitro Microsystem Brain Penetration Preclinical Assays Blood Brain Barrier Phenotype Cell-cell Communication Molecular Analysis Healthy And Pathological Conditions Molecule And Particle Transports Inter And Intracellular Trafficking Poly-L-Lysine Solution Astrocytes 1X PBS CMF Trypsin EDTA Solution
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