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Chapters
- 00:04Introduction
- 01:23Seeding of Astrocytes in the Wells
- 02:50Seeding of Pericytes on the Reverted Insert Filters
- 04:35Seeding of Endothelial Cells on the Insert Filters
- 06:00Maintenance of the Triple Cell Culture for the Induction of the BBB Properties
- 06:35Results: Properties of the Triple Culture BBB Model and the Comparison of BLEC Gene Expression and Protein Level of Distinctive Markers in the Triple Culture Model with the Co‐culture BBB Model
- 08:49Conclusion
This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
