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Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
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Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Article DOI: 10.3791/63225-v • 07:04 min • February 9th, 2022
February 9th, 2022 •
1, 1, 2, 1, 1
1Department of Cell Biology and Physiology and Center for the Investigation of Membrane Excitability Diseases, Washington University in St. Louis, 2Department of Genetics, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University

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Summary
Automatic Translation

The present protocol describes the acute isolation of viable cardiac and vascular smooth muscle cells from adult, juvenile, larval, and embryonic zebrafish (Danio rerio), suitable for electrophysiological studies.

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Isolation Cardiac Muscle Cells Vascular Smooth Muscle Cells Zebrafish Electrophysiological Studies Patch Clamp Electrophysiology Native Ion Channel Properties Perfusion Buffer Dissecting Microscope Pectoral Muscles Fins Atrium Ventricle Bulbus Arteriosus Ventral Aorta Sinus Venosus Cardiomyocytes
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