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Chemical Cartography Approaches to Study Trypanosomatid Infection
This content is Free Access.
1Department of Chemistry and Biochemistry,University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research,University of Oklahoma, Norman, 3Department of Anthropology,University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology,University of Oklahoma, Norman
Chapters
- 00:04Introduction
- 00:55Tissue Collection and Metabolite Extraction
- 02:563D Model Generation
- 04:55‘ili Plot Generation
- 07:04Results: 3D Model of Metabolite Distribution During Trypanosomatid Infection
- 07:37Conclusion
This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization.
