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JoVE Journal
Bioengineering

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Light-Controlled Fermentations for Microbial Chemical and Protein Production
 

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Article DOI: 10.3791/63269-v 08:37 min March 22nd, 2022
March 22nd, 2022

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Summary

Optogenetic control of microbial metabolism offers flexible dynamic control over fermentation processes. The protocol here shows how to set up blue light-regulated fermentations for chemical and protein production at different volumetric scales.

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Tags

Optogenetics Light-controlled Fermentations Microbial Chemical Production Protein Production Titers Inducers Light-based Control Optimization Three-phase Fermentations Multi-chromatic Control Saccharomyces Cerevisiae Strain OptoINVRT Plasmids Gene Deletion Strain Construction Linearize Plasmid PIGA One Promoter Lithium Acetate Transformation Methods HIS3 Auxotrophic Strain
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