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Light-Controlled Fermentations for Microbial Chemical and Protein Production
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Bioengineering
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JoVE Journal Bioengineering
Light-Controlled Fermentations for Microbial Chemical and Protein Production

Light-Controlled Fermentations for Microbial Chemical and Protein Production

DOI:
10.3791/63269-v

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08:37 min

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March 22, 2022

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, ,
1Department of Chemical and Biological Engineering,Princeton University, 2The Andlinger Center for Energy and the Environment,Princeton University, 3Department of Molecular Biology,Princeton University, 4High Meadows Environmental Institute,Princeton University

Chapters

  • 00:04Introduction
  • 00:43Light-Controlled Chemical Production Using the Saccharomyces cerevisiae OptoINVRT7 Circuit
  • 05:12Three-Phase Fermentation Using the Saccharomyces cerevisiae OptoAMP System
  • 06:26Results: The Effects of Light-Regulated Fermentations for Chemical and Protein Production
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Optogenetic control of microbial metabolism offers flexible dynamic control over fermentation processes. The protocol here shows how to set up blue light-regulated fermentations for chemical and protein production at different volumetric scales.

Tags

Keywords: OptogeneticsLight-controlled FermentationMicrobial Chemical ProductionMicrobial Protein ProductionSaccharomyces CerevisiaeOptoINVRTPIGA1 PromoterBlue LightGenetic EngineeringBioprocessingBiomanufacturing

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