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JoVE Journal
Bioengineering

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自发荧光成像评估细胞代谢
 
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自发荧光成像评估细胞代谢

Article DOI: 10.3791/63282-v 07:36 min November 15th, 2021
November 15th, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

该协议描述了内源性代谢辅酶,还原烟酰胺腺嘌呤(磷酸盐)二核苷酸(NAD(P)H)和氧化黄素腺嘌呤二核苷酸(FAD)的荧光成像和分析。NAD(P)H 和 FAD 的自发荧光成像提供了一种无标记、无损的方法来评估细胞代谢。

Tags

生物工程,第177期,FLIM,代谢,NAD(P)H,FAD,荧光,显微镜
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