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JoVE Journal
Bioengineering

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Autofluorescensbilleddannelse til evaluering af cellulær metabolisme
 
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Autofluorescensbilleddannelse til evaluering af cellulær metabolisme

Article DOI: 10.3791/63282-v 07:36 min November 15th, 2021
November 15th, 2021

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Summary

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Denne protokol beskriver fluorescensbilleddannelse og analyse af de endogene metaboliske coenzymer, reduceret nicotinamid adenin (phosphat) dinukleotid (NAD (P) H) og oxideret flavin adenin dinukleotid (FAD). Autofluorescensbilleddannelse af NAD (P) H og FAD giver en etiketfri, ikke-destruktiv metode til vurdering af cellulær metabolisme.

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Bioengineering udgave 177 FLIM Metabolisme NAD(P)H FAD Fluorescens Mikroskopi
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