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Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

DOI:
10.3791/63291-v

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08:55 min

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December 13, 2021

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,
1Department of Biology,University of Rochester

Chapters

  • 00:05Introduction
  • 00:40Injection Needle Preparation
  • 01:26Injection Solution Preparation and Needle Loading
  • 02:08Embryo Preparation for Microinjection
  • 04:34Microinjecting Embryos
  • 05:51Imaging Embryos
  • 06:38Results: Representative Results for Organelle Dependent Trafficking in Drosophila Embryos
  • 08:14Conclusion

Summary

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In the early Drosophila embryo, many organelles are motile. In principle, they can be imaged live via specific fluorescent probes, but the eggshell prevents direct application to the embryo. This protocol describes how to introduce such probes via microinjection, and then analyze bulk organelle motion via particle image velocimetry.

Tags

Keywords: CytoskeletonTraffickingLipid-containing OrganellesDrosophila EmbryosFluorescent DyesFluorescent ProbesCell CultureGenetic BackgroundInjection NeedlesCapillary HolderNeedle PullerDissecting MicroscopeInjection SolutionHeptane GlueHalocarbon OilChorionEmbryo StageDouble-sided Tape

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