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Journal / Developmental Biology / Differenzierung humaner pluripotenter Stammzellen …
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Developmental Biology

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JoVE Journal Developmental Biology
Differenzierung humaner pluripotenter Stammzellen zu Betazellvorläufern der Bauchspeicheldrüse in einem 2D-Kultursystem
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Journal (Developmental Biology)
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Automated Cell Culture System Settings for Maintenance and Differentiation of Human Pluripotent Stem Cells (Video) | JoVE
Journal (Developmental Biology)
Automated Cell Culture System Settings for Maintenance and Differentiation of Human Pluripotent Stem Cells (Video) | JoVE
 
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Differenzierung humaner pluripotenter Stammzellen zu Betazellvorläufern der Bauchspeicheldrüse in einem 2D-Kultursystem

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2
1College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

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Das vorliegende Protokoll beschreibt eine verbesserte Methode zur Erhöhung der Koexpression von PDX1- und NKX6.1-Transkriptionsfaktoren in Pankreas-Vorläuferzellen aus humanen pluripotenten Stammzellen (hPS-Zellen) in planaren Monoschichten. Dies wird erreicht, indem die frische Matrix aufgefüllt, die Zelldichte manipuliert und die endodermalen Zellen dissoziiert werden.

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Entwicklungsbiologie Ausgabe 178
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