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JoVE Journal Neuroscience
Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
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Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Article DOI: 10.3791/63308-v • 11:12 min • December 1st, 2021
December 1st, 2021 •
1, 1, 1,2, 3, 1,2
1Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies, Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology, Kazan State Medical University

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Summary
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The protocol describes the method of loading a fluorescent calcium dye through the cut nerve into mouse motor nerve terminals. In addition, a unique method for recording fast calcium transients in the peripheral nerve endings using confocal microscopy is presented.

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Registration Calcium Transients Mouse Neuromuscular Junction High Temporal Resolution Confocal Microscopy Presynaptic Calcium Level Synaptic Transmission Calcium Signaling Neurodegenerative Diseases Loading Technique Staining Spatial-temporal Resolution Neurophysiologists Fast Periodic Processes Confocal Microscope Pipette Holding Levator Auris Longus Muscle Tissue Elastomer Coated Petri Dish Ringer's Solution Intracellular Recordings Micropipette Puller
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