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Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
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Neuroscience
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JoVE Journal Neuroscience
Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

DOI:
10.3791/63313-v

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14:45 min

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July 29, 2022

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, , , , , ,
1Laboratorio de Neuroinmunología,Fundación Ciencia & Vida, 2Center for Integrative Biology,Universidad Mayor, 3FONDAP Geroscience Center for Brain Health and Metabolism, 4Buck Institute for Research on Ageing, 5Facultad de Medicina y Ciencia,Universidad San Sebastián

Chapters

  • 00:13Introduction
  • 02:25The Stereotaxic Surgery
  • 05:38Determination of the Motor Performance Using the Beam Test
  • 07:00Tissue Processing
  • 08:51Representative Results
  • 12:59Conclusion

Summary

Automatic Translation

Automatic Translation

This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.

Tags

Keywords: Parkinson’s DiseaseMouse ModelAdeno-associated Viral VectorsHuman α-synucleinStereotaxic DeliveryNigrostriatal PathwayNeurodegenerationNeuroinflammationMotor ImpairmentSynuclein PathologyMicroglial ActivationT-cell ActivationAseptic EnvironmentAnalgesiaAnesthesia

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